Three new antisera with high sensitivity to ecdysone, 3-dehydroecdysone and other A-ring derivatives [Elektronisk resurs] Production and characterization
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Von Gliscynski, Ulla (författare)
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Delbeque, Jean Paul (författare)
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Böcking, Detlef (författare)
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Sedlmeier, Dietrich (författare)
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Dircksen, Heinrich 1954- (författare)
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Lafont, René (författare)
- 1995
- Engelska.
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Ingår i: European Journal of Entomology. - 1210-5759. ; 92:1, 75-79
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Sammanfattning
Ämnesord
Stäng
- Three new antisera have been produced in rabbits immunized with E-2/3-hemisuccinate conjugates. In a competitive enzyme immunoassay (EIA) with 20-ecdysone-peroxidase as a tracer, the antisera exhibit high specificity towards the side chain of the ecdysone (E) molecule. Modifications of the A-ring do result in no or small changes of sensitivity. One antiserum (RUD-2) allows the direct quantification of total ecdysteroids in samples containing E and 3-dehydroecdysone in varying ratios, without further sophisticated sample treatment. At least one of the other antisera allows the direct quantification of mixtures of E and 25-deoxyecdysone (RUD-1). Thus, these antisera may be a useful tool in studying molting control in arthropods.
Ämnesord
- Natural Sciences (hsv)
- Biological Sciences (hsv)
- Zoology (hsv)
- Naturvetenskap (hsv)
- Biologiska vetenskaper (hsv)
- Zoologi (hsv)
- zoologi (su)
- Zoology (su)
Indexterm och SAB-rubrik
- Antisera
- ecdysone
- ecdysteroid
- EIA
- enzyme immunoassay
- molt
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