Immunoassays on thiol-ene synthetic paper generate a superior fluorescence signal [Elektronisk resurs]

• The fluorescence-based detection of biological complexes on solid substrates is widely used in microarrays and lateral flow tests. Here, we investigate thiol-ene micropillar scaffold sheets (“synthetic paper”) as the solid substrate in such assays. Compared to state-of-the-art glass and nitrocellulose substrates, assays on synthetic paper provide a stronger fluorescence signal, similar or better reproducibility, lower limit of detection (LOD), and the possibility of working with lower immunoreagent concentrations. Using synthetic paper, we detected the antibiotic enrofloxacin in whole milk with a LOD of 1.64 nM, which is on par or better than the values obtained with other common tests, and much lower than the maximum level allowed by European Union regulations. The significance of these results lays in that they indicate that synthetically-derived microstructured substrate materials have the potential to improve the performance of diagnostic assays. 

Ämnesord

Engineering and Technology  (hsv)

Chemical Engineering  (hsv)

Polymer Technologies  (hsv)

Teknik och teknologier  (hsv)

Kemiteknik  (hsv)

Polymerteknologi  (hsv)

Electrical Engineering, Electronic Engineering, Information Engineering  (hsv)

Other Electrical Engineering, Electronic Engineering, Information Engineering  (hsv)

Elektroteknik och elektronik  (hsv)

Annan elektroteknik och elektronik  (hsv)

Materials Engineering  (hsv)

Paper, Pulp and Fiber Technology  (hsv)

Materialteknik  (hsv)

Pappers-, massa- och fiberteknik  (hsv)

Mechanical Engineering  (hsv)

Fluid Mechanics and Acoustics  (hsv)

Maskinteknik  (hsv)

Strömningsmekanik och akustik  (hsv)

Biotechnology  (kth)

Bioteknologi  (kth)

Electrical Engineering  (kth)

Elektro- och systemteknik  (kth)

Kemi  (kth)

Chemistry  (kth)

Genre

government publication  (marcgt)

Indexterm och SAB-rubrik

Biosensor; Enrofloxacin; Fluorescence; Glass slides; Microarray; Nitrocellulose; Off-stoichiometric thiol-ene; Porous substrate