Melittin–Lipid interaction [Elektronisk resurs] A comparative study using liposomes, micelles and bilayer disks

• Comparison of melittin interaction with liposomes, bilayer disks and micelles showed that melittin binding to lipid aggregates is largely dictated by the amount of highly curved areas in the aggregates. The PEG-stabilised bilayer disks were characterised by a combination of small angle neutron scattering, cryo-transmission electron microscopy and dynamic light scattering. Importantly, the theoretically foreseen partial segregation of the lipid components, important for maintaining the structure of the bilayer disk, was confirmed. Steady state fluorescence spectroscopy indicated that melittin mainly resides at the rim of the bilayer disks. Results of the present study help increase the understanding of the mechanisms behind, and the physico-chemical factors affecting, melittin–lipid interaction. We suggest that bilayer disks, due to their stable structure, constitute interesting vehicles for transport of peptides that have high propensity to associate with lipid surfaces of high curvature. 

Ämnesord

Natural Sciences  (hsv)

Chemical Sciences  (hsv)

Physical Chemistry  (hsv)

Naturvetenskap  (hsv)

Kemi  (hsv)

Fysikalisk kemi  (hsv)

NATURAL SCIENCES  (svep)

Chemistry  (svep)

Physical chemistry  (svep)

NATURVETENSKAP  (svep)

Kemi  (svep)

Fysikalisk kemi  (svep)

Fysikalisk kemi  (uu)

Physical Chemistry  (uu)

Materialvetenskap  (uu)

Materials Science  (uu)

Indexterm och SAB-rubrik

Bilayer disk

PEG–lipid

Micelle

Liposome

Neutron scattering

Melittin