Gold allergy : in vitro studies using peripheral blood mononuclear cells / Jenny Clifford.
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Clifford, Jenny, 1980- (författare)
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- Linköpings universitet. Institutionen för klinisk och experimentell medicin (utgivare)
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Alternativt namn: Linköping University. Department of Clinical and Experimental Medicine
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Alternativt namn: Linköping University. Faculty of Health Sciences. Department of Clinical and Experimental Medicine
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Alternativt namn: IKE
- Publicerad: Linköping : Department of Clinical and Experimental Medicine, Linköping University, 2009
- Engelska 1 onlineresurs (67 sidor)
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Serie: Linköping studies in health sciences. Thesis, 1100-6013 ; 102
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Läs hela texten (Sammanfattning och ramberättelse från Linköping University Electronic Press)
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Sammanfattning
Ämnesord
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- Positive patch test reactions to gold are commonly seen in dermatology clinics, but it is very unusual for the patients to actually have any clinical symptoms. It is also common with irritant reactions that are not linked to adaptive immunity. Therefore, a deeper understanding of the mechanisms underlying allergic contact dermatitis (ACD) reaction, and the search for a complementing diagnostic tool, is important. In paper I we included three subject groups; one with morphologically positive patch test reactions to gold sodium thiosulphate (GSTS, the gold salt used in patch testing), one with negative patch tests, and one with irritant reactions to gold. Blood samples were collected and examined regarding the proliferation rate and which cytokines were secreted after culturing with GSTS. We saw that the cultured lymphocytes from the allergic donors proliferated at a significantly higher rate than the two other subject groups, and that the cells secreted cytokines of both Th1 (Interferon (IFN) -g and Interleukin (IL) -2) and Th2 (IL-13 and IL-10) types. The allergic donors secreted significantly higher levels of IFN-g, IL-2 and IL-13 than the two othersubject groups. Both the negative and irritant subject groups showed suppressed levels of the cytokines as compared with the unstimulated cultures, demonstrating the immunosuppressing effects of gold. We also examined whether any of the analyzed markers, alone or combined, could be used as an aid for diagnosing ACD to gold. We found that the IFN-g assay yielded the highestsensitivity (81.8 %) and specificity (82.1 %), and also identified 87.5 % of the irritant group as non-allergic. In paper II we decided to investigate what cell types and subsets that reacted to the gold stimulation. We analyzed proliferation rate and expression of CD45RA, CD45R0, cutaneouslymphocyte-associated antigen (CLA) and the chemokine receptors CXCR3, CCR4 andCCR10. Similar to what has previously been published about nickel (Ni) allergy, the cells from the gold-allergic subjects that reacted to the GSTS stimulation expressed CD3+CD4+CD45R0+CLA+. However, contrary to findings in studies on Ni-reactive cells, we saw no differences between allergic and non-allergic subjects regarding any of the chemokine receptors studied. In conclusion, we found that analysis of IFN-g might be a useful complement to patchtesting, possibly of interest in avoiding the need for repeated tests to rule out irritant reactions. We also saw that the cells that proliferated in response to gold were memory T-cells expressing CD4 and CLA, the marker for skin-homing. However, these cells did not express elevated levels of any of the chemokine receptors analyzed, showing that there are both similarities and differences between the mechanisms for Ni allergy and gold allergy.
Ämnesord
- Allergi (sao)
- Guld (sao)
- Allergy and Immunology (MeSH)
- Medical and Health Sciences (ssif)
- Clinical Medicine (ssif)
- Dermatology and Venereal Diseases (ssif)
- Medicin och hälsovetenskap (ssif)
- Klinisk medicin (ssif)
- Dermatologi och venereologi (ssif)
- MEDICINE (svep)
- Dermatology and venerology,clinical genetics, internal medicine (svep)
- Dermatology and venerology (svep)
- MEDICIN (svep)
- Dermatologi och venerologi, klinisk genetik, invärtesmedicin (svep)
- Dermatologi och venerologi (svep)
- Allergy (LCSH)
Genre
- government publication (marcgt)
Indexterm och SAB-rubrik
- Vad Immunologi
Klassifikation
- 616.973 (DDC)
- Vad (kssb/8)
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