Microfluidic system for the identification of bacterial pathogens causing urinary tract infections [Elektronisk resurs]
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Becker, Holger (författare)
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Conference on Microfluidics, BioMEMS, and Medical Microsystems XIII, FEB 07-09, 2015, San Francisco, CA, United States
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Hlawatsch, Nadine (författare)
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Haraldsson, Tommy (författare)
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van der Wijngaart, Wouter (författare)
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Lind, Anders (författare)
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Malhotra-Kumar, Surbi (författare)
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Turlej-Rogacka, Agata (författare)
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Goossens, Herman (författare)
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KTH Skolan för elektro- och systemteknik (EES) (utgivare)
- Publicerad: SPIE - International Society for Optical Engineering, 2015
- Engelska.
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Serie: Proceedings of SPIE, 0277-786X
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Ingår i: Microfluidics, BioMEMS, and Medical Microsystems XIII.
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Sammanfattning
Ämnesord
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- Urinary tract infections (UTIs) are among the most common bacterial infections and pose a significant healthcare burden. The growing trend in antibiotic resistance makes it mandatory to develop diagnostic kits which allow not only the determination of a pathogen but also the antibiotic resistances. We have developed a microfluidic cartridge which takes a direct urine sample, extracts the DNA, performs an amplification using batch-PCR and flows the sample over a microarray which is printed into a microchannel for fluorescence detection. The cartridge is injection-molded out of COP and contains a set of two-component injection-molded rotary valves to switch between input and to isolate the PCR chamber during thermocycling. The hybridization probes were spotted directly onto a functionalized section of the outlet microchannel. We have been able to successfully perform PCR of E. coli in urine in this chip and perform a fluorescence detection of PCR products. An upgraded design of the cartridge contains the buffers and reagents in blisters stored on the chip.
Ämnesord
- Engineering and Technology (hsv)
- Other Engineering and Technologies (hsv)
- Teknik och teknologier (hsv)
- Annan teknik (hsv)
Indexterm och SAB-rubrik
- Lab-on-a-chip
- Point-of-care diagnostics
- microfluidics
- PCR
- urinary tract infections
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