The effects of bisphenol A and bisphenol S on adipokine expression and glucose metabolism in human adipose tissue [Elektronisk resurs]

• Purpose The environmental endocrine disruptors, bisphenol A (BPA) and bisphenol S (BPS) are associated with the development of type 2 diabetes. We aim to study the effects of BPA or BPS exposure on adipokine expression in human adipose tissue and on adipocyte glucose uptake. Methods Human subcutaneous adipose tissue was treated for 24 or 72 h with environmentally-relevant and supraphysiological concentrations of BPA or BPS (1–10 4 nM). Following exposure, gene expression of proinflammatory cytokines, adipokines, and estrogen receptors was measured in adipose tissue. Glucose uptake and the insulin signalling pathway were analyzed in isolated adipocytes following adipose tissue culture with BPA for 24 h. Results Adipose tissue treated with BPA for 24 h had reduced expression of the proinflammatory genes ( IL6 , IL1B, TNFA) and adipokines ( ADIPOQ, FABP4 ). BPA and BPS had no effect on the expression of other proinflammatory genes ( IL33 ), adipokines ( LEP ), or receptors ( ESR1, ESR2 ) after 72-h exposure. Adipose tissue treated with environmentally-relevant concentrations of BPA for 24 h had reduced insulin-stimulated glucose uptake, without altered gene and protein levels of key insulin signalling pathway markers. Conclusions We found that human adipose tissue treated with environmentally-relevant concentrations of BPA for 24 h, but not BPS, reduced expression of proinflammatory genes and adipokines. Furthermore, BPA reduced glucose uptake in adipocytes independently of insulin signalling. Such mechanisms can contribute to the development of insulin resistance associated with BPA exposure. 

Ämnesord

Medical and Health Sciences  (hsv)

Clinical Medicine  (hsv)

Endocrinology and Diabetes  (hsv)

Medicin och hälsovetenskap  (hsv)

Klinisk medicin  (hsv)

Endokrinologi och diabetes  (hsv)

Medical and Health Sciences  (hsv)

Basic Medicine  (hsv)

Pharmacology and Toxicology  (hsv)

Medicin och hälsovetenskap  (hsv)

Medicinska och farmaceutiska grundvetenskaper  (hsv)

Farmakologi och toxikologi  (hsv)

Genre

government publication  (marcgt)

Indexterm och SAB-rubrik

Bisphenol

Adipose tissue

Inflammation

Glucose metabolism

Insulin signalling