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Development of methods for determining aflatoxins in biological material [Elektronisk resurs]

Kussak, Anders, 1964- (författare)
Stephany, Rainer (opponent)
Umeå universitet Teknisk-naturvetenskapliga fakulteten (utgivare)
Publicerad: Umeå : Umeå universitet, 1995
Engelska 51
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  • Bok (digitaliserad)Avhandling(Diss. (sammanfattning) Umeå : Umeå universitet, 1995)
Sammanfattning Ämnesord
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  • In this thesis, it is shown how aflatoxins can be determined in biological material. The thesis is a summary of five papers. Aflatoxins are carcinogenic mycotoxins produced by Aspergillus moulds. Methods were developed for the determination of aflatoxins in samples of airborne dust and human urine collected at feed factories. For the dust samples from such agricultural products as copra, cotton seed and maize, methods were developed for the determination of aflatoxins B 1 , B 2 , G 1 and G 2 . For urine samples, methods were developed for analysing the four aflatoxins above that naturally occur in dust, and the metabolites aflatoxins M 1 and Q 1 . Sample preparation of dust samples included solvent extraction, filtration and immunoaffinity column extraction. Urine samples were cleaned up using immunoaffinity column extraction or solid-phase extraction using ethyl bonded-phase columns. All extractions with these columns were automated by means of a laboratory robot. Reversed-phase liquid chromatography was used to separate the aflatoxins in the cleaned-up extracts. Detection was performed by fluorescence after post-column derivatization by addition of bromine. Parameters for the derivatization were studied using factorial designs. To confirm the identity of aflatoxins in naturally contaminated airborne dust samples and spiked urine, liquid chromatography was combined with electrospray mass spectrometry. The detection limits of the aflatoxins in dust samples were in the range 1.8-3.1 ng/g in 10-mg dust samples using fluorescence detection. Aflatoxins were determined in spiked urine down to the 6.8-18 pg/ml level. In naturally contaminated dust of copra and cotton seed, aflatoxins were detected with a content of 9-50 pg/mg of aflatoxin Bi. No aflatoxins could be detected in any urine sample obtained from feed factory workers that were less than 6.8 pg/ml of aflatoxins B 1 , B 2 , G 1 and G 2 and less than 18 pg/ml of aflatoxins M 1 and Q 1 . 

Ämnesord

Natural Sciences  (ssif)
Chemical Sciences  (ssif)
Analytical Chemistry  (ssif)
Naturvetenskap  (ssif)
Kemi  (ssif)
Analytisk kemi  (ssif)

Genre

government publication  (marcgt)

Indexterm och SAB-rubrik

Aflatoxins
Mycotoxins
Carcinogen
Airborne dust
Urine
Feed factories
Immunoaffinity extraction
Solid-phase extraction
Post-column derivatization
Electrospray
Mass spectrometry
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