Recombinant antibody against Trypanosoma cruzi from patients with chronic Chagas heart disease recognizes mammalian nervous system [Elektronisk resurs]
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Niborski, Leticia L. (författare)
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Potenza, Mariana (författare)
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Chirivi, Renato G. S. (författare)
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Simonetti, Leandro (författare)
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Ossowski, Micaela S. (författare)
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Grippo, Vanina (författare)
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May, Maria (författare)
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Staquicini, Daniela (författare)
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Parodi-Talice, Adriana (författare)
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Robello, Carlos (författare)
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Comini, Marcelo A. (författare)
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Alonso, Guillermo D. (författare)
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Raats, Jos M. H. (författare)
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Gomez, Karina A. (författare)
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Uppsala universitet Teknisk-naturvetenskapliga vetenskapsområdet (utgivare)
- Publicerad: ELSEVIER, 2021
- Engelska.
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Ingår i: EBioMedicine. ; 63
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- Background: To deeply understand the role of antibodies in the context of Trypanosoma cruzi infection, we decided to characterize A2R1, a parasite antibody selected from single-chain variable fragment (scFv) phage display libraries constructed from B cells of chronic Chagas heart disease patients. Methods: Immunoblot, ELISA, cytometry, immunofluorescence and immunohistochemical assays were used to characterize A2R1 reactivity. To identify the antibody target, we performed an immunoprecipitation and two-dimensional electrophoresis coupled to mass spectrometry and confirmed A2R1 specific interaction by producing the antigen in different expression systems. Based on these data, we carried out a comparative in silico analysis of the protein targets orthologues, focusing mainly on post-translational modifications. Findings: A2R1 recognizes a parasite protein of similar to 50 kDa present in all life cycle stages of T. cruzi, as well as in other members of the kinetoplastid family, showing a defined immunofluorescence labeling pattern consistent with the cytoskeleton. A2R1 binds to tubulin, but this interaction relies on its post-translational modifications. Interestingly, this antibody also targets mammalian tubulin only present in brain, staining in and around cell bodies of the human peripheral and central nervous system. Interpretation: Our findings demonstrate for the first time the existence of a human antibody against T. cruzi tubulin capable of cross-reacting with a human neural protein. This work re-emphasizes the role of molecular mimicry between host and parasitic antigens in the development of pathological manifestations of T. cruzi infection.
Ämnesord
- Natural Sciences (hsv)
- Biological Sciences (hsv)
- Biochemistry and Molecular Biology (hsv)
- Naturvetenskap (hsv)
- Biologiska vetenskaper (hsv)
- Biokemi och molekylärbiologi (hsv)
Genre
- government publication (marcgt)
Indexterm och SAB-rubrik
- Chagas disease
- Phage-display
- Tubulin
- Molecular mimicry
- Post-translational modification
- Digestive system
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EBioMedicine