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Differentiation of human dermal fibroblasts and applications in tissue engineering / Pehr Sommar.

Sommar, Pehr, 1975- (författare)
Kratz, Gunnar (preses)
Hansson, Thomas (preses)
Lindahl, Anders (opponent)
Laboratory for Reconstructive Plastic Surgery (medarbetare)
Linköpings universitet Institutionen för klinisk och experimentell medicin (utgivare)
Linköpings universitet Hälsouniversitetet (utgivare)
Publicerad: Linköping : Linköping University Electronic Press, 2010
Engelska 1 onlineresurs (62 sidor)
Serie: Linköping University Medical Dissertations, 0345-0082 0345-0082 ; 1202
Läs hela texten (Sammanfattning och ramberättelse från Linköping University Electronic Press)
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  • E-bokAvhandling(Diss. (sammanfattning) Linköping : Linköpings universitet, 2010)
Sammanfattning Ämnesord
Stäng  
  • Tissue engineering applies principles of biology and engineering to the development of functional substitutes for damaged or lost tissues. Tools for the neo-generation of tissue in tissue engineering research include cells, biomaterials and soluble factors. One main obstacle in tissue engineering is the limited availability of autologous tissue specific progenitor cells. This has led to interest into using autologous cells with stem cell plasticity. Bone marrow derived stem cells were the first adult stem cells shown to have multilineage potential. Since, several reports have been published indicating that cells from other tissues; fat, muscle, connective tissue e.g., possess potential to differentiate into lineages distinct from their tissue of origin. The optimal cell type for use in tissue engineering applications should be easy to obtain, cultivate and store. The human dermal fibroblast is an easily accessible cell source, which after routine cell expansion gives a substantial cell yield from a small skin biopsy. Hence, the dermal fibroblast could be a suitable cell source for tissue engineering applications.The main aim of this thesis was to investigate the differentiation capacity of human dermal fibroblasts, and their possible applications in bone and cartilage tissue engineering applications. 
  • Human dermal fibroblasts were shown to differentiate towards adipogenic, chondrogenic, and osteogenic phenotypes upon subjection to specific induction media. Differentiation was seen both in unrefined primary cultures and in clonal populations (paper I). Fibroblasts could be used to create three-dimensional cartilage- and bone like tissue when grown in vitro on gelatin microcarriers in combination with platelet rich plasma (paper II). 4 weeks after in vivo implantation of osteogenic induced fibroblasts into a fracture model in athymic rats, dense cell clusters and viable human cells were found in the gaps, but no visible healing of defects as determined by CT-scanning (paper III). After the induction towards adipogenic, chondrogenic, endotheliogenic and osteogenic lineages, gene expression analysis by microarray and quantitative real-time-PCR found several master regulatory genes important for lineage commitment, as well as phenotypically relevant genes regulated as compared to reference cultures (paper IV). In conclusion, results obtained in this thesis suggest an inherent ability for controllable phenotype alteration of human dermal fibroblasts in vitro. We conclude that dermal fibroblasts could be induced towards adipogenic, chondrogenic, endotheliogenic or osteogenic novel phenotypes which suggest a genetic readiness of differentiated fibroblasts for lineage-specific biological functionality, indicating that human dermal fibroblasts might be a suitable cell source in tissue engineering applications. 

Ämnesord

Bone and bones  (MeSH)
Cartilage  (MeSH)
Cell differentiation  (MeSH)
Clone cells  (MeSH)
Adipocytes  -- cytology (MeSH)
Chondrocytes  -- cytology (MeSH)
Dermis  -- cytology (MeSH)
Fibroblasts  -- cytology (MeSH)
Osteogenesis  -- physiology (MeSH)
Tissue engineering  -- methods (MeSH)
Medical and Health Sciences  (ssif)
Clinical Medicine  (ssif)
Surgery  (ssif)
Medicin och hälsovetenskap  (ssif)
Klinisk medicin  (ssif)
Kirurgi  (ssif)
MEDICINE  (svep)
Surgery  (svep)
Surgical research  (svep)
Plastic surgery  (svep)
MEDICIN  (svep)
Kirurgi  (svep)
Kirurgisk forskning  (svep)
Plastikkirurgi  (svep)

Genre

government publication  (marcgt)

Indexterm och SAB-rubrik

Tissue Engineering
Human Dermal Fibroblasts
Differentiation
Adipogenesis
Chondrogenesis
Endotheliogenesis
Osteogenesis
Vff Transplantation

Klassifikation

617.954 (DDC)
Vff (kssb/8)
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