1. Introduction -- 2. Literature review -- The ensiling process -- Carbohydrates associated with grass silage -- Micro-organisms associated with grass silage -- Effects of organic acids on silage micro-organisms -- Effects of carbon dioxide (CO2) on silage micro-organisms -- Silage additives -- Losses during ensiling -- 3. Aims of the study -- 4. Materials and methods -- Experimental design -- Chemical methods -- Microbiological methods -- Fermentation gas losses and aerobic stability -- Statistical methods -- 5. Results -- Isolation and characterization of LAB from silage (Experiment 1) -- Selection of a heterofermentative inoculant (Experiment 2) -- Influence of additives on silage quality (experiment 3) -- Influence of additives on silage quality (experiment 4) -- Influence of additives on silage quality (experiment 5) -- Influence of additives on silage quality (experiment 6) -- Influence of additives on silage quality (experiment 7) -- Influence of additives on silage quality (experiment 8) -- The lactic acid:acetic acid ratio -- The quality of silage according to DLG-scheme -- Losses of DM during ensiling -- Aerobic stability -- The effect of the heterofermentative inoculant on silage quality -- Relationship between pH and silage quality -- Relationship between aerobic stability and silage quality -- Epiphytic LAB -- 6. Discussion -- Isolation of LAB from silages (Experiment 1) -- Effect of two L. brevis variants on silage quality (Experiment 2) -- Influence of additives on silage quality (Experiment 3) -- Influence of additives on wilted grass silage quality (Experiments 4 – 8) -- Losses of DM during ensiling -- Aerobic stability -- Influence of the heterofermentative inoculant on silage quality -- Epiphytic LAB -- 7. Concluding remarks.
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