Size characterization of inclusion bodies by sedimentation field-flow fractionation [Elektronisk resurs]
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Margreiter, Gerd (författare)
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Messner, Paul (författare)
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Caldwell, Karin D (författare)
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Bayer, Karl (författare)
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Ytbioteknik (medarbetare)
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Uppsala universitet Teknisk-naturvetenskapliga vetenskapsområdet (utgivare)
- Publicerad: 2008
- Engelska.
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Ingår i: Journal of Biotechnology. - 0168-1656. ; 138:3-4, 67-73
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Sammanfattning
Ämnesord
Stäng
- Sedimentation field-flow fractionation (sedFFF) was evaluated to characterize the size of Delta(4-23)TEM-beta-lactamase inclusion bodies (IBs) overexpressed in fed-batch cultivations of Escherichia coli. Heterologous Delta(4-23)TEM-beta-lactamase protein formed different sizes of IBs, depending upon the induction conditions. In the early phases of recombinant protein expression, induced with low concentrations of IPTG (isopropyl-beta-d-thiogalactoside), IB masses were larger than expected and showed heterogeneous size distributions. During cultivation, IB sizes showed a Gaussian distribution and reached a broad range by the end of the fed-batch cultivations. The obtained result proved the aptitude of sedFFF to rapidly assess the size distribution of IBs in a culture.
Ämnesord
- Natural Sciences (hsv)
- Chemical Sciences (hsv)
- Naturvetenskap (hsv)
- Kemi (hsv)
- NATURAL SCIENCES (svep)
- Chemistry (svep)
- NATURVETENSKAP (svep)
- Kemi (svep)
Genre
- government publication (marcgt)
Indexterm och SAB-rubrik
- Sedimentation field-flow fractionation
- Inclusion bodies
- β-Lactamase; Fed-batch cultivation
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Journal of Biotechnology