Bioanalytics : analytical methods and concepts in biochemistry and molecular biology / edited by Friedrich Lottspeich, Joachim Engels.

• PART 1. PROTEIN ANALYTICS -- 1 Protein Purification -- 2 Protein determination -- 3 Enzyme Activity Testing -- 4 Microcalorimetry -- 5 Immunological Techniques -- 6 Chemical Modification of Proteins and Protein Complexes -- 7 Spectroscopy -- 8 Light Microscopy Techniques : Imaging -- 9 Cleavage of Proteins -- 10 Chromatographic Separation Methods -- 11 Electrophoretic Techniques -- 12 Capillary Electrophoresis -- 13 Amino Acid Analysis -- 14 Protein Sequence Analysis -- 15 Mass Spectrometry -- 16 Protein–Protein Interactions -- 17 Biosensors -- PART 2. 3D STRUCTURE DETERMINATION -- 18 Magnetic Resonance Spectroscopy of Biomolecules -- 19 Electron Microscopy -- 20 Atomic Force Microscopy -- 21 X-Ray Structure Analysis -- PART 3. PEPTIDES, CARBOHYDRATES, AND LIPIDS -- 22 Analytics of Synthetic Peptides -- 23 Carbohydrate Analysis -- 24 Lipid Analysis -- 25 Analysis of Post-translational Modifications: Phosphorylation and Acetylation of Proteins -- PART 4. NUCLEIC ACID ANALYTICS -- 26 Isolation and Purification of Nucleic Acids -- 27 Analysis of Nucleic Acids -- 28 Techniques for the Hybridization and Detection of Nucleic Acids -- 29 Polymerase Chain Reaction -- 30 DNA Sequencing -- 31 Analysis of Epigenetic Modifications -- 32 Protein–Nucleic Acid Interactions -- PART 5. FUNCTIONAL AND SYSTEM ANALYTICS -- 33 Sequence Data Analysis -- 34 Analysis of Promoter Strength and Nascent RNA Synthesis -- 35 Fluorescent In Situ Hybridization in Molecular Cytogenetics -- 36 Physical and Genetic Mapping of Genomes -- 37 DNA-Microarray Technology -- 38 The Use of Oligonucleotides as Tools in Cell Biology -- 39 Proteome Analysis -- 40 Metabolomics and Peptidomics -- 41 Interactomics : Systematic Protein–Protein Interactions -- 42 Chemical Biology -- 43 Toponome Analysis -- Appendix 1: Amino Acids and Posttranslational Modifications -- Appendix 2: Symbols and Abbreviations -- Appendix 3: Standard Amino Acids (three and one letter code) -- Appendix 4: Nucleic Acid Bases. 

• Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. 

Ämnesord

Analytical biochemistry.  (LCSH)

Genre

Klassifikation

572.36 (DDC)

Ue.0482 (kssb/8 (machine generated))