Synthetic Paper Separates Plasma from Whole Blood with Low Protein Loss [Elektronisk resurs]
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GUO, WEIJIN (författare)
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Hansson, Jonas (författare)
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van der Wijngaart, Wouter (författare)
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Biomicrofluidics (medarbetare)
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Biomicrofluidics (medarbetare)
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Biomicrofluidics (medarbetare)
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KTH Skolan för elektroteknik och datavetenskap (EECS) (utgivare)
- Publicerad: Washington D.C. American Chemical Society (ACS), 2020
- Engelska.
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Ingår i: Analytical Chemistry. - 0003-2700.
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Sammanfattning
Ämnesord
Stäng
- The separation of plasma from whole blood is the first step in many diagnostic tests. Point-of-care tests often rely on integrated plasma filters, but protein retention in such filters limits their performance. Here, we investigate plasma separation on interlocked micropillar scaffolds ("synthetic paper") by the local agglutination of blood cells coupled with the capillary separation of the plasma. We separated clinically relevant volumes of plasma with high efficiency in a separation time on par with that of state of the art techniques. We investigated different covalent and non-covalent surface treatments (PEGMA, HEMA, BSA, O2 plasma) on our blood filter and their effect on protein recovery, and identified O2 plasma treatment and 7.9 μg/cm2 agglutination antibody as most suitable treatments. Using these treatments, we recovered at least 82% of the blood plasma proteins, more than with state-of-the-art filters. The simplicity of our device and the performance of our approach could enable better point-of-care tests.
Ämnesord
- Engineering and Technology (hsv)
- Medical Engineering (hsv)
- Medical Materials (hsv)
- Teknik och teknologier (hsv)
- Medicinteknik (hsv)
- Medicinska material och protesteknik (hsv)
- Industrial Biotechnology (hsv)
- Bio Materials (hsv)
- Industriell bioteknik (hsv)
- Biomaterial (hsv)
- Materials Engineering (hsv)
- Paper, Pulp and Fiber Technology (hsv)
- Materialteknik (hsv)
- Pappers-, massa- och fiberteknik (hsv)
- Electrical Engineering, Electronic Engineering, Information Engineering (hsv)
- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv)
- Elektroteknik och elektronik (hsv)
- Annan elektroteknik och elektronik (hsv)
- Kemi (kth)
- Chemistry (kth)
- Biotechnology (kth)
- Bioteknologi (kth)
- Electrical Engineering (kth)
- Elektro- och systemteknik (kth)
Genre
- government publication (marcgt)
Indexterm och SAB-rubrik
- capillary
- blood
- plasma separation
- synthetic paper
- protein recovery rate
- OSTE
Inställningar
Hjälp
Ingår i annan publikation. Gå till titeln
Analytical Chemistry